Plasma Protein Binding Free Drug Assay
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by: CathyHigginson3
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Word Count: 511
Date: Mon, 28 Nov 2011 Time: 10:01 PM
How does plasma protein binding influence the effect of a drug on the body?
Plasma protein binding free drug assay is an important aspect of how a particular drug influences the body and manifests its curative properties. When a drug is meant to act only on a particular part of the body, plasma protein binding free drug assay is used. In this form, the drug is confined to the particular region because it is bound. If a drug is unbound, then it spread through various membranes and cells in the body and acts on all parts, enzymes, ions and receptors. Binding can be done in different degrees. Higher the binding of the drug to the plasma protein, lower will be its ability to impact a larger part of the body and subsequently, higher will be its effect on the part of the body where it is supposed to act. In other words, the extent to which the binding takes place has a lot of influence on the distribution of the drug in the body as well as its elimination. Distribution is larger in unbound drugs. The half life of the drug is also higher when it is bounded. Clearance or elimination of the drug, however, is not subject to binding.
What are the important features in the assay?
The assay can be use single or unique plasma proteins, besides using the entire plasma. In addition, it is possible to use plasma not only from the human beings but from different creatures as well. There are different kinds of assays available. They help in different purposes. You can use them for prioritizing the compound series or just the compounds in the initial stages. It is also possible to make out the extent of binding and single out plasma proteins that are active in binding the compound.
Information about the assay
As mentioned above, the effect of a drug on the human body is largely dependent on the extent of binding of the drug. The drug is distributed in the body based on its binding. However, care has to be taken when binding is formulated because extensive binding can inhibit the function of the drug and lower the rate at which it acts on the body, ability of the body to metabolize the drug and its elimination from the body. One of the ways in which assessment of binding of the plasma protein is possible is by equilibrium dialysis. It is considered to be one of the best ways of accessing the details of binding of the drug. The choice of the assay is dependent on the characteristics of the compound and on the available budget.
What is the use of plasma protein binding free drug assay?
The effect of a drug on the body largely depends on its pharmaceutical properties, one of the important of which is the concept of binding. In order to evaluate the binding and its effects on the body, several methods such as ultracentrifugation and ultra filtration are used besides equilibrium dialysis, which is the most commonly used method.
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